Derivatizing Reagents

These reagents are manufactured by UCT to exact standards

of purity and consistency.

Product Recommendations: 

Derivatizing reagents are sold bottled in airtight septum vials blanketed with nitrogen to maintain their activity.  Contact with moisture can greatly reduce reactivity.  Protect from air and moisture.  Keep bottle tightly sealed when not in use.

 

!    It is critical that pipettes, glassware, vials, etc.    !

be free of water or alcohol before derivatization.

Benefits of Derivatization: 

  • Improved chromatographic resolution - increased volatility - reduced intermolecular hydrogen bonding - separation of structurally similar compounds.
  • Improved mass spectral characteristics - higher mass fragments - greater S/N (signal to noise ratio) - more unique masses - increased abundance/sensitivity of molecular ions.
  • Improved thermal stability of some compounds - reduced thermal degradation - higher temperatures to speed analysis.
  • Increased instrument and lab productivity - fewer reinjections or repeats due to peak tailing - high confidence in analyte identification and quantitation - easy to perform - inert by-products of derivatization will not degrade capillary column performance.